Antilisterial activity of dromedary lactoferrin peptic hydrolysates.
Identifieur interne : 000560 ( Main/Exploration ); précédent : 000559; suivant : 000561Antilisterial activity of dromedary lactoferrin peptic hydrolysates.
Auteurs : Zeineb Jrad [Tunisie] ; Halima El-Hatmi [Tunisie] ; Isabelle Adt [France] ; Sandrine Gouin [France] ; Julien Jardin [France] ; Olfa Oussaief [Tunisie] ; Mohamed Dbara [Tunisie] ; Samira Arroum [Tunisie] ; Touhami Khorchani [Tunisie] ; Pascal Degraeve [France] ; Nadia Oulahal [France]Source :
- Journal of dairy science [ 1525-3198 ] ; 2019.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Antibactériens (métabolisme), Antibactériens (pharmacologie), Bovins (MeSH), Chameaux (MeSH), Femelle (MeSH), Fragments peptidiques (MeSH), Hydrolyse (MeSH), Lactoferrine (métabolisme), Lactoferrine (pharmacologie), Lait (composition chimique), Listeria (effets des médicaments et des substances chimiques), Pepsine A (métabolisme), Peptides (métabolisme), Peptides (pharmacologie), Séquence d'acides aminés (MeSH).
- MESH :
- composition chimique : Lait.
- effets des médicaments et des substances chimiques : Listeria.
- métabolisme : Antibactériens, Lactoferrine, Pepsine A, Peptides.
- pharmacologie : Antibactériens, Lactoferrine, Peptides.
- Animaux, Bovins, Chameaux, Femelle, Fragments peptidiques, Hydrolyse, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Animals (MeSH), Anti-Bacterial Agents (metabolism), Anti-Bacterial Agents (pharmacology), Camelus (MeSH), Cattle (MeSH), Female (MeSH), Hydrolysis (MeSH), Lactoferrin (metabolism), Lactoferrin (pharmacology), Listeria (drug effects), Milk (chemistry), Pepsin A (metabolism), Peptide Fragments (MeSH), Peptides (metabolism), Peptides (pharmacology).
- MESH :
- chemical , metabolism : Anti-Bacterial Agents, Lactoferrin, Pepsin A, Peptides.
- chemical , pharmacology : Anti-Bacterial Agents, Lactoferrin, Peptides.
- chemistry : Milk.
- drug effects : Listeria.
- Amino Acid Sequence, Animals, Camelus, Cattle, Female, Hydrolysis, Peptide Fragments.
Abstract
The aim of this study was to explore the antibacterial peptides derived from dromedary lactoferrin (LFc). The LFc was purified from colostrum using a batch procedure with a cation exchange chromatography support and was hydrolyzed with pepsin to generate peptic digest. This peptic digest was fractionated by cation exchange chromatography, and the antilisterial activity of LFc, peptic digest, and obtained fractions was investigated using the bioscreen method. The growth of Listeria innocua ATCC 33090 and LRGIA 01 strains was not inhibited by LFc and its hydrolysates. Two fractions of dromedary lactoferrin peptic hydrolysate were active against both strains. A tandem mass spectroscopy analysis revealed that the 2 active fractions comprised at least 227 different peptides. Among these peptides, 9 found in the first fraction had at least 50% similarity with 10 known antimicrobial peptides (following sequence alignments with the antimicrobial peptide database from the University of Nebraska Medical Center, Omaha). Whereas 9 of these peptides presented homology with honeybee, frog, or amphibian peptides, the 10th peptide, F152SASCVPCVDGKEYPNLCQLCAGTGENKCACSSQEPYFGY192 (specifically found in 1 separated fraction), exibited 54% homology with a synthetic antibacterial peptide (AP00481) derived from human lactoferrin named kaliocin-1. Similarly, the second fraction contained 1 peptide similar to lactoferrampin B, an antibacterial peptide derived from bovine milk. This result suggests that peptic hydrolysis of LFc releases more active antimicrobial peptides than their protein source and thus provides an opportunity for their potential use to improve food safety by inhibiting undesirable and spoilage bacteria.
DOI: 10.3168/jds.2018-15548
PubMed: 31005319
Affiliations:
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Interfaces Alimentaires), Equipe Mixte d'Accueil no. 3733, IUT Lyon 1, 01000 Bourg en Bresse, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>University of Claude Bernard Lyon 1, ISARA Lyon, BioDyMIA (Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires), Equipe Mixte d'Accueil no. 3733, IUT Lyon 1, 01000 Bourg en Bresse</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Bourg-en-Bresse</settlement></placeName></affiliation></author><author><name sortKey="Oulahal, Nadia" sort="Oulahal, Nadia" uniqKey="Oulahal N" first="Nadia" last="Oulahal">Nadia Oulahal</name><affiliation wicri:level="3"><nlm:affiliation>University of Claude Bernard Lyon 1, ISARA Lyon, BioDyMIA (Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires), Equipe Mixte d'Accueil no. 3733, IUT Lyon 1, 01000 Bourg en Bresse, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>University of Claude Bernard Lyon 1, ISARA Lyon, BioDyMIA (Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires), Equipe Mixte d'Accueil no. 3733, IUT Lyon 1, 01000 Bourg en Bresse</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Bourg-en-Bresse</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31005319</idno><idno type="pmid">31005319</idno><idno type="doi">10.3168/jds.2018-15548</idno><idno type="wicri:Area/PubMed/Corpus">000258</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000258</idno><idno type="wicri:Area/PubMed/Curation">000257</idno><idno type="wicri:explorRef" wicri:stream="PubMed" 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sort="El Hatmi, Halima" uniqKey="El Hatmi H" first="Halima" last="El-Hatmi">Halima El-Hatmi</name><affiliation wicri:level="1"><nlm:affiliation>Livestock and Wildlife Laboratory, Arid Land Institute, 4119, Medenine, Tunisia; Food Department, Higher Institute of Applied Biology of Médenine, University of Gabes, 4119, Medenine, Tunisia. Electronic address: halima.elhatmi@ira.rnrt.tn.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Livestock and Wildlife Laboratory, Arid Land Institute, 4119, Medenine, Tunisia; Food Department, Higher Institute of Applied Biology of Médenine, University of Gabes, 4119, Medenine</wicri:regionArea><wicri:noRegion>Medenine</wicri:noRegion></affiliation></author><author><name sortKey="Adt, Isabelle" sort="Adt, Isabelle" uniqKey="Adt I" first="Isabelle" last="Adt">Isabelle Adt</name><affiliation wicri:level="3"><nlm:affiliation>University of Claude Bernard Lyon 1, ISARA Lyon, BioDyMIA (Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires), Equipe Mixte d'Accueil no. 3733, IUT Lyon 1, 01000 Bourg en Bresse, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>University of Claude Bernard Lyon 1, ISARA Lyon, BioDyMIA (Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires), Equipe Mixte d'Accueil no. 3733, IUT Lyon 1, 01000 Bourg en Bresse</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Bourg-en-Bresse</settlement></placeName></affiliation></author><author><name sortKey="Gouin, Sandrine" sort="Gouin, Sandrine" uniqKey="Gouin S" first="Sandrine" last="Gouin">Sandrine Gouin</name><affiliation wicri:level="3"><nlm:affiliation>University of Claude Bernard Lyon 1, ISARA Lyon, BioDyMIA (Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires), Equipe Mixte d'Accueil no. 3733, IUT Lyon 1, 01000 Bourg en Bresse, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>University of Claude Bernard Lyon 1, ISARA Lyon, BioDyMIA (Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires), Equipe Mixte d'Accueil no. 3733, IUT Lyon 1, 01000 Bourg en Bresse</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Bourg-en-Bresse</settlement></placeName></affiliation></author><author><name sortKey="Jardin, Julien" sort="Jardin, Julien" uniqKey="Jardin J" first="Julien" last="Jardin">Julien Jardin</name><affiliation wicri:level="3"><nlm:affiliation>STLO, UMR1253, INRA, AgroCampus Ouest, 35000 Rennes, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>STLO, UMR1253, INRA, AgroCampus Ouest, 35000 Rennes</wicri:regionArea><placeName><region type="region" nuts="2">Région Bretagne</region><settlement type="city">Rennes</settlement></placeName></affiliation></author><author><name sortKey="Oussaief, Olfa" sort="Oussaief, Olfa" uniqKey="Oussaief O" first="Olfa" last="Oussaief">Olfa Oussaief</name><affiliation wicri:level="1"><nlm:affiliation>Livestock and Wildlife Laboratory, Arid Land Institute, 4119, Medenine, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Livestock and Wildlife Laboratory, Arid Land Institute, 4119, Medenine</wicri:regionArea><wicri:noRegion>Medenine</wicri:noRegion></affiliation></author><author><name sortKey="Dbara, Mohamed" sort="Dbara, Mohamed" uniqKey="Dbara M" first="Mohamed" last="Dbara">Mohamed Dbara</name><affiliation wicri:level="1"><nlm:affiliation>Livestock and Wildlife Laboratory, Arid Land Institute, 4119, Medenine, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Livestock and Wildlife Laboratory, Arid Land Institute, 4119, Medenine</wicri:regionArea><wicri:noRegion>Medenine</wicri:noRegion></affiliation></author><author><name sortKey="Arroum, Samira" sort="Arroum, Samira" uniqKey="Arroum S" first="Samira" last="Arroum">Samira Arroum</name><affiliation wicri:level="1"><nlm:affiliation>Livestock and Wildlife Laboratory, Arid Land Institute, 4119, Medenine, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Livestock and Wildlife Laboratory, Arid Land Institute, 4119, Medenine</wicri:regionArea><wicri:noRegion>Medenine</wicri:noRegion></affiliation></author><author><name sortKey="Khorchani, Touhami" sort="Khorchani, Touhami" uniqKey="Khorchani T" first="Touhami" last="Khorchani">Touhami Khorchani</name><affiliation wicri:level="1"><nlm:affiliation>Livestock and Wildlife Laboratory, Arid Land Institute, 4119, Medenine, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Livestock and Wildlife Laboratory, Arid Land Institute, 4119, Medenine</wicri:regionArea><wicri:noRegion>Medenine</wicri:noRegion></affiliation></author><author><name sortKey="Degraeve, Pascal" sort="Degraeve, Pascal" uniqKey="Degraeve P" first="Pascal" last="Degraeve">Pascal Degraeve</name><affiliation wicri:level="3"><nlm:affiliation>University of Claude Bernard Lyon 1, ISARA Lyon, BioDyMIA (Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires), Equipe Mixte d'Accueil no. 3733, IUT Lyon 1, 01000 Bourg en Bresse, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>University of Claude Bernard Lyon 1, ISARA Lyon, BioDyMIA (Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires), Equipe Mixte d'Accueil no. 3733, IUT Lyon 1, 01000 Bourg en Bresse</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Bourg-en-Bresse</settlement></placeName></affiliation></author><author><name sortKey="Oulahal, Nadia" sort="Oulahal, Nadia" uniqKey="Oulahal N" first="Nadia" last="Oulahal">Nadia Oulahal</name><affiliation wicri:level="3"><nlm:affiliation>University of Claude Bernard Lyon 1, ISARA Lyon, BioDyMIA (Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires), Equipe Mixte d'Accueil no. 3733, IUT Lyon 1, 01000 Bourg en Bresse, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>University of Claude Bernard Lyon 1, ISARA Lyon, BioDyMIA (Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires), Equipe Mixte d'Accueil no. 3733, IUT Lyon 1, 01000 Bourg en Bresse</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Bourg-en-Bresse</settlement></placeName></affiliation></author></analytic><series><title level="j">Journal of dairy science</title><idno type="eISSN">1525-3198</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Animals (MeSH)</term><term>Anti-Bacterial Agents (metabolism)</term><term>Anti-Bacterial Agents (pharmacology)</term><term>Camelus (MeSH)</term><term>Cattle (MeSH)</term><term>Female (MeSH)</term><term>Hydrolysis (MeSH)</term><term>Lactoferrin (metabolism)</term><term>Lactoferrin (pharmacology)</term><term>Listeria (drug effects)</term><term>Milk (chemistry)</term><term>Pepsin A (metabolism)</term><term>Peptide Fragments (MeSH)</term><term>Peptides (metabolism)</term><term>Peptides (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Antibactériens (métabolisme)</term><term>Antibactériens (pharmacologie)</term><term>Bovins (MeSH)</term><term>Chameaux (MeSH)</term><term>Femelle (MeSH)</term><term>Fragments peptidiques (MeSH)</term><term>Hydrolyse (MeSH)</term><term>Lactoferrine (métabolisme)</term><term>Lactoferrine (pharmacologie)</term><term>Lait (composition chimique)</term><term>Listeria (effets des médicaments et des substances chimiques)</term><term>Pepsine A (métabolisme)</term><term>Peptides (métabolisme)</term><term>Peptides (pharmacologie)</term><term>Séquence d'acides aminés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Lactoferrin</term><term>Pepsin A</term><term>Peptides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Lactoferrin</term><term>Peptides</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Milk</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Lait</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Listeria</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Listeria</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antibactériens</term><term>Lactoferrine</term><term>Pepsine A</term><term>Peptides</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antibactériens</term><term>Lactoferrine</term><term>Peptides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Camelus</term><term>Cattle</term><term>Female</term><term>Hydrolysis</term><term>Peptide Fragments</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Bovins</term><term>Chameaux</term><term>Femelle</term><term>Fragments peptidiques</term><term>Hydrolyse</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of this study was to explore the antibacterial peptides derived from dromedary lactoferrin (LFc). The LFc was purified from colostrum using a batch procedure with a cation exchange chromatography support and was hydrolyzed with pepsin to generate peptic digest. This peptic digest was fractionated by cation exchange chromatography, and the antilisterial activity of LFc, peptic digest, and obtained fractions was investigated using the bioscreen method. The growth of Listeria innocua ATCC 33090 and LRGIA 01 strains was not inhibited by LFc and its hydrolysates. Two fractions of dromedary lactoferrin peptic hydrolysate were active against both strains. A tandem mass spectroscopy analysis revealed that the 2 active fractions comprised at least 227 different peptides. Among these peptides, 9 found in the first fraction had at least 50% similarity with 10 known antimicrobial peptides (following sequence alignments with the antimicrobial peptide database from the University of Nebraska Medical Center, Omaha). Whereas 9 of these peptides presented homology with honeybee, frog, or amphibian peptides, the 10th peptide, F<sub>152</sub>SASCVPCVDGKEYPNLCQLCAGTGENKCACSSQEPYFGY<sub>192</sub> (specifically found in 1 separated fraction), exibited 54% homology with a synthetic antibacterial peptide (AP00481) derived from human lactoferrin named kaliocin-1. Similarly, the second fraction contained 1 peptide similar to lactoferrampin B, an antibacterial peptide derived from bovine milk. This result suggests that peptic hydrolysis of LFc releases more active antimicrobial peptides than their protein source and thus provides an opportunity for their potential use to improve food safety by inhibiting undesirable and spoilage bacteria.</div></front></TEI><affiliations><list><country><li>France</li><li>Tunisie</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li><li>Région Bretagne</li></region><settlement><li>Bourg-en-Bresse</li><li>Rennes</li></settlement></list><tree><country name="Tunisie"><noRegion><name sortKey="Jrad, Zeineb" sort="Jrad, Zeineb" uniqKey="Jrad Z" first="Zeineb" last="Jrad">Zeineb Jrad</name></noRegion><name sortKey="Arroum, Samira" sort="Arroum, Samira" uniqKey="Arroum S" first="Samira" last="Arroum">Samira Arroum</name><name sortKey="Dbara, Mohamed" sort="Dbara, Mohamed" uniqKey="Dbara M" first="Mohamed" last="Dbara">Mohamed Dbara</name><name sortKey="El Hatmi, Halima" sort="El Hatmi, Halima" uniqKey="El Hatmi H" first="Halima" last="El-Hatmi">Halima El-Hatmi</name><name sortKey="Khorchani, Touhami" sort="Khorchani, Touhami" uniqKey="Khorchani T" first="Touhami" last="Khorchani">Touhami Khorchani</name><name sortKey="Oussaief, Olfa" sort="Oussaief, Olfa" uniqKey="Oussaief O" first="Olfa" last="Oussaief">Olfa Oussaief</name></country><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Adt, Isabelle" sort="Adt, Isabelle" uniqKey="Adt I" first="Isabelle" last="Adt">Isabelle Adt</name></region><name sortKey="Degraeve, Pascal" sort="Degraeve, Pascal" uniqKey="Degraeve P" first="Pascal" last="Degraeve">Pascal Degraeve</name><name sortKey="Gouin, Sandrine" sort="Gouin, Sandrine" uniqKey="Gouin S" first="Sandrine" last="Gouin">Sandrine Gouin</name><name sortKey="Jardin, Julien" sort="Jardin, Julien" uniqKey="Jardin J" first="Julien" last="Jardin">Julien Jardin</name><name sortKey="Oulahal, Nadia" sort="Oulahal, Nadia" uniqKey="Oulahal N" first="Nadia" last="Oulahal">Nadia Oulahal</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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